Applicability of a sensitive duplex real-time PCR assay for identifying B/Yamagata and B/Victoria lineages of influenza virus from clinical specimens
Identifieur interne : 000D53 ( Main/Exploration ); précédent : 000D52; suivant : 000D54Applicability of a sensitive duplex real-time PCR assay for identifying B/Yamagata and B/Victoria lineages of influenza virus from clinical specimens
Auteurs : Naixing Zhang [République populaire de Chine] ; Shisong Fang [République populaire de Chine] ; Ting Wang [République populaire de Chine] ; Jianxiong Li [République populaire de Chine] ; Xiaowen Cheng [République populaire de Chine] ; Cunyou Zhao [République populaire de Chine] ; Xin Wang [République populaire de Chine] ; Xing Lv [République populaire de Chine] ; Chunli Wu [République populaire de Chine] ; Renli Zhang [République populaire de Chine] ; Jinquan Cheng [République populaire de Chine] ; Hong Xue [République populaire de Chine] ; Zuxun Lu [République populaire de Chine]Source :
- Applied Microbiology and Biotechnology [ 0175-7598 ] ; 2012-01-01.
English descriptors
Abstract
Abstract: Type B influenza virus is one of the major epidemic strains and responsible for considerable mortality and morbidity. Rapidly and accurately identifying different influenza B virus lineages, i.e., B/Yamagata (B/Y) and B/Victoria (B/V), is desirable during the flu season. However, the available rapid techniques lack sensitivity, and the usual methods for identifying influenza viruses require expansion of virus in tissue culture or embryonated hen’s eggs. Thus, we developed several sets of primer pairs that were able to detect and distinguish B/Y and B/V in a single real-time PCR assay. Used in conjunction with two sets of specific primers that exhibited purine at 3′ end of at least one primer targeting on HA gene of B/Y and B/V lineages allows us to accurately identify approximately 102 copies per microliter for B/Y and B/V with intra- and inter-assay coefficient of variation (CV) <4%. When it was used to test 17,765 throat swab specimens obtained in the 2006–2010 influenza surveillance season, this method was comparable to hemagglutination inhibition assay in detection, typing and subtyping of influenza viruses with 100% true-negative (specificity) and 100% true-positive (sensitivity). Taken together, this method provides sensitive and robust tool for routine diagnosis and on-time epidemiological examination for WHO decisions on vaccine composition.
Url:
DOI: 10.1007/s00253-011-3710-8
Affiliations:
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to="805">805</biblScope></imprint><idno type="ISSN">0175-7598</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0175-7598</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Influenza virus</term><term>Real-time RT-PCR</term><term>Victoria lineage</term><term>Yamagata lineage</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Type B influenza virus is one of the major epidemic strains and responsible for considerable mortality and morbidity. Rapidly and accurately identifying different influenza B virus lineages, i.e., B/Yamagata (B/Y) and B/Victoria (B/V), is desirable during the flu season. However, the available rapid techniques lack sensitivity, and the usual methods for identifying influenza viruses require expansion of virus in tissue culture or embryonated hen’s eggs. Thus, we developed several sets of primer pairs that were able to detect and distinguish B/Y and B/V in a single real-time PCR assay. Used in conjunction with two sets of specific primers that exhibited purine at 3′ end of at least one primer targeting on HA gene of B/Y and B/V lineages allows us to accurately identify approximately 102 copies per microliter for B/Y and B/V with intra- and inter-assay coefficient of variation (CV) <4%. When it was used to test 17,765 throat swab specimens obtained in the 2006–2010 influenza surveillance season, this method was comparable to hemagglutination inhibition assay in detection, typing and subtyping of influenza viruses with 100% true-negative (specificity) and 100% true-positive (sensitivity). Taken together, this method provides sensitive and robust tool for routine diagnosis and on-time epidemiological examination for WHO decisions on vaccine composition.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li><li>Hubei</li><li>Jiangxi</li></region><settlement><li>Jiangmen</li><li>Nanchang</li><li>Shenzhen</li><li>Wuhan</li></settlement></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Zhang, Naixing" sort="Zhang, Naixing" uniqKey="Zhang N" first="Naixing" last="Zhang">Naixing Zhang</name></region><name sortKey="Cheng, Jinquan" sort="Cheng, Jinquan" uniqKey="Cheng J" first="Jinquan" last="Cheng">Jinquan Cheng</name><name sortKey="Cheng, Xiaowen" sort="Cheng, Xiaowen" uniqKey="Cheng X" first="Xiaowen" last="Cheng">Xiaowen Cheng</name><name sortKey="Fang, Shisong" sort="Fang, Shisong" uniqKey="Fang S" first="Shisong" last="Fang">Shisong Fang</name><name sortKey="Fang, Shisong" sort="Fang, Shisong" uniqKey="Fang S" first="Shisong" last="Fang">Shisong Fang</name><name sortKey="Fang, Shisong" sort="Fang, Shisong" uniqKey="Fang S" first="Shisong" last="Fang">Shisong Fang</name><name sortKey="Li, Jianxiong" sort="Li, Jianxiong" uniqKey="Li J" first="Jianxiong" last="Li">Jianxiong Li</name><name sortKey="Lu, Zuxun" sort="Lu, Zuxun" uniqKey="Lu Z" first="Zuxun" last="Lu">Zuxun Lu</name><name sortKey="Lv, Xing" sort="Lv, Xing" uniqKey="Lv X" first="Xing" last="Lv">Xing Lv</name><name sortKey="Wang, Ting" sort="Wang, Ting" uniqKey="Wang T" first="Ting" last="Wang">Ting Wang</name><name sortKey="Wang, Xin" sort="Wang, Xin" uniqKey="Wang X" first="Xin" last="Wang">Xin Wang</name><name sortKey="Wu, Chunli" sort="Wu, Chunli" uniqKey="Wu C" first="Chunli" last="Wu">Chunli Wu</name><name sortKey="Xue, Hong" sort="Xue, Hong" uniqKey="Xue H" first="Hong" last="Xue">Hong Xue</name><name sortKey="Zhang, Renli" sort="Zhang, Renli" uniqKey="Zhang R" first="Renli" last="Zhang">Renli Zhang</name><name sortKey="Zhao, Cunyou" sort="Zhao, Cunyou" uniqKey="Zhao C" first="Cunyou" last="Zhao">Cunyou Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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